Molecular monitoring of the Leu-164 mutation of dihydrofolate reductase in a highly sulfadoxine/pyrimethamine-resistant area in Africa. Journal Article


Authors: Ochong, Edwin; Nzila, Alexis; Kimani, Serah; Kokwaro, Gilbert; Mutabingwa, Theonest; Watkins, William; Marsh, Kevin
Article Title: Molecular monitoring of the Leu-164 mutation of dihydrofolate reductase in a highly sulfadoxine/pyrimethamine-resistant area in Africa.
Keywords: AFRICA; MALARIA; DRUG resistance; PLASMODIUM falciparum; ANIMAL mutation; PLASMODIUM
Journal Title: Malaria Journal
Volume: 2
ISSN: 14752875
Publisher: BioMed Central  
Date Published: 2003-01
Start Page: 46
End Page: 3
DOI/URL:
Notes: --- - Article - The selection of point mutation at codon 164 (from isoleucine to leucine) of the dihydrofolate reductase (DHFR) enzyme in Plasmodium falciparum is associated with high sulfadoxine / pyrimethamine (SP) resistance. Using the yeast expression system that allows the detection of dhfr allele present at low level, the presence of this mutation had previously been reported between 1998-1999 in Muheza, Tanzania, an area of high SP resistance. Eighty five P. falciparum isolates, obtained from the same area between 2002 and 2003, were analysed for the presence of Leu-164 mutation, using standard protocol based on PCR-RFLP. None of the isolates had the Leu-164 mutation. [ABSTRACT FROM AUTHOR] - Copyright of Malaria Journal is the property of BioMed Central and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) - ! 'Accession Number: 30095604; Ochong, Edwin 1; Email Address: eochong@wtnairobi.mimcom.net Nzila, Alexis 1,2; Email Address: anzila@wtnairobi.mimcom.net Kimani, Serah 1; Email Address: skimani@wtnairobi.mimcom.net Kokwaro, Gilbert 1; Email Address: GKokwaro@wtnairobi.mimcom.net Mutabingwa, Theonest 3,4; Email Address: tkmuta@ud.co.tz Watkins, William 2; Email Address: bwatkins@btinternet.com Marsh, Kevin 5,6; Email Address: kmarsh@kilifi.mimcom.net; Affiliation: 1: Kenya Medical Research Institute (KEMRI)/Wellcome Trust Collaborative Research Programme, Wellcome Trust Research Laboratories P.O Box 43640 Nairobi, Kenya 2: Department of Pharmacology and Therapeutics, University of Liverpool, Liverpool L69 3BX, UK 3: National Institute for Medical Research, Amani, Tanzania 4: London School of Tropical Medicine and Hygiene, Gates Malaria Partnership, London, UK 5: KEMRI/Wellcome Trust Collaborative Research Programme, Centre for Geographical Medicine Research KEMRI PO Box 230, Kilifi, Kenya 6: Nuffield Department of Medicine, John Radcliffe Hospital, Oxford, UK; Source Info: 2003, Vol. 2, p46; Subject Term: ANIMAL mutation; Subject Term: PLASMODIUM; Subject Term: PLASMODIUM falciparum; Subject Term: MALARIA; Subject Term: DRUG resistance; Subject Term: AFRICA; Number of Pages: 3p; Document Type: Article'
SU Authors
  1. Prof. Gilbert Kokwaro
    26 Kokwaro